Mane Six

The Mane Six, also known as the Elements of Harmony are the protagonist group of My Little Pony: Friendship is Magic. The group consists of Princess Twilight Sparkle (main protagonist), Pinkie Pie, Applejack, Rainbow Dash, Rarity, and Fluttershy (the other five protagonists). While not being one of the elements, Spike is also in the group and their good friend, being one of Twilight's closest childhood friends and her assistant 

Trivia:

On Your Marks, is the first episode when all Mane Six and Spike did not appear in.